Phenotypically Varying P270 Protein of Type II T. vaginalis isolates (C20A3, 270-kDa)

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  • Monoclonal antibody to a major surface immunogen differentiates isolates and subpopulations of Trichomonas vaginalis.

  • MAb led to the discovery of two distinct isolate types of T. vaginalis: heterogeneous phenotypically-varying (PV) Type II and homogeneous, non-phenotypically varying Type I.

  • The discovery that non-fluorescent Type II isolate organisms and non-fluorescent Type I organisms adhered and killed host epithelial cells. The fluorescent organisms express P270 on surface and are non-adherent.

  • The discovery that the MAb C20A3 showed 95+ percent of in vivo trichomonads from women were negative phenotype (no P270 on surface of parasites)

  • The discovery that only phenotypically varying Type II detected by MAb C20A3 were infected with dsRNA viruses.

  • The discovery that it was a “repertoire” of high molecular weight proteins that underwent phenotypic variation in a coordinated fashion.

  • The discovery that the protein P270 had many repeated protein segments, each containing the identical DREGRD epitope detected by C20A3.

  • The discovery that the phenotypic variation was under the control of iron.

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  • Monoclonal antibody to a major surface immunogen differentiates isolates and subpopulations of Trichomonas vaginalis.

  • MAb led to the discovery of two distinct isolate types of T. vaginalis: heterogeneous phenotypically-varying (PV) Type II and homogeneous, non-phenotypically varying Type I.

  • The discovery that non-fluorescent Type II isolate organisms and non-fluorescent Type I organisms adhered and killed host epithelial cells. The fluorescent organisms express P270 on surface and are non-adherent.

  • The discovery that the MAb C20A3 showed 95+ percent of in vivo trichomonads from women were negative phenotype (no P270 on surface of parasites)

  • The discovery that only phenotypically varying Type II detected by MAb C20A3 were infected with dsRNA viruses.

  • The discovery that it was a “repertoire” of high molecular weight proteins that underwent phenotypic variation in a coordinated fashion.

  • The discovery that the protein P270 had many repeated protein segments, each containing the identical DREGRD epitope detected by C20A3.

  • The discovery that the phenotypic variation was under the control of iron.

  • Monoclonal antibody to a major surface immunogen differentiates isolates and subpopulations of Trichomonas vaginalis.

  • MAb led to the discovery of two distinct isolate types of T. vaginalis: heterogeneous phenotypically-varying (PV) Type II and homogeneous, non-phenotypically varying Type I.

  • The discovery that non-fluorescent Type II isolate organisms and non-fluorescent Type I organisms adhered and killed host epithelial cells. The fluorescent organisms express P270 on surface and are non-adherent.

  • The discovery that the MAb C20A3 showed 95+ percent of in vivo trichomonads from women were negative phenotype (no P270 on surface of parasites)

  • The discovery that only phenotypically varying Type II detected by MAb C20A3 were infected with dsRNA viruses.

  • The discovery that it was a “repertoire” of high molecular weight proteins that underwent phenotypic variation in a coordinated fashion.

  • The discovery that the protein P270 had many repeated protein segments, each containing the identical DREGRD epitope detected by C20A3.

  • The discovery that the phenotypic variation was under the control of iron.